Department of Biochemistry and Molecular Biology (BMB)

Center for Genomics Research

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Overview

The Center for Genomics Research (CGR) is a core research facility of the Boonshoft School of Medicine and the College of Science and Mathematics at Wright State University. CGR's primary mission is to provide support and core facilities for Wright State research faculty engaged in basic and clinical research in the areas of gene expression, flow cytometry and genotyping. The center strives to cultivate collaborations between basic and clinical researchers at Wright State through training of personnel, collaborative projects undertaken at CGR and joint grant submissions involving researchers and CGR members.

The CGR is open to all Wright State-affiliated faculty. CGR charges investigator grants for use of certain equipment to offset the costs for contracts, reagents and staff time required for equipment maintenance. For groups not associated with WSU, the center works on genomic research projects on a fee-for-service basis. For more information about CGR use and services, contact Dr. Michael Markey: (phone: (937) 775-4536; email: michael.markey@wright.edu.)


Collaborate with CGR

What can CGR do for my project?

CGR has collaborated on projects at Wright State and external to the university.  CGR can help in the design of experiments, provide expertise and "hands" for performing experiments, perform data analysis and interpretation, and utilize facilities for quantitative PCR, flow cytometry, microarrays, and next generation sequencing.  Study topics may include:

  • Gene expression
  • Chromosomal copy number changes
  • Single nucleotide polymorphism genotyping
  • Exon and splice junction usage
  • DNA next generation sequencing
    • Structural variants
    • Point mutations
    • Copy number variation
    • Exome sequencing
    • Targeted sequencing
    • Whole genome sequencing
  • RNA sequencing (RNA-seq)
    • Gene expression
    • Gene fusion
    • Splice variants
    • Transcriptome sequencing
    • Targeted RNA-seq
  • Chromatin immunoprecipitation sequencing (ChIP-seq)
  • Methylation sequencing (Methyl-seq)
  • Flow cytometry
    • Cell cycle analysis
    • Surface marker expression
  • Other methods in genomics

When should I contact CGR?

CGR can be most effective if involved from the beginning of a project.  CGR can consult during the grant writing process and provide letters of support.  It is preferred that CGR be written into grant-funded research as a collaborator.  For questions or begin the process, contact Michael Markey at (937) 775-4536.


Staff

Michael P. Markey, PhD

Department:
Biochemistry/Molecular Biology-SOM
Title:
Research Associate Professor, Biochemistry & Molecular Biology; Director, Center for Genomics Research
Address:
Diggs Laboratory 048, 3640 Colonel Glenn Hwy., Dayton, OH 45435-0001

Madhavi Kadakia, PhD

Department:
Biochemistry/Molecular Biology-SOM
Title:
Chair and Professor, Biochemistry & Molecular Biology; Associate Dean for Research Affairs, BSOM
Address:
Diggs Laboratory 122, 3640 Colonel Glenn Hwy., Dayton, OH 45435-0001

Core Resources

Resource Applications
ABI 2700 thermocycler PCR and other thermocycling
ABI 7900 HT SDS with PCR clean room TaqMan based RT-PCR
SNP genotyping
Syber green based RT-PCR
ABI GeneAmp 9700 thermocycler PCR and other thermocycling
ABI QuantStudio 7 thermocycler Real-time PCR and Array Cards
ABI StepOne thermocycler Real-time PCR

Accuri C6 Flow Cytometer

Flow cytometric analyses

Affymetrix GeneChip System Gene expression profiling
Global human genotyping
Agilent Bioanalyzer 2100 RNA, DNA and protein quantification

Amersham 600

Captures images; chemiluminescence; ethidium bromide; white light images

Fuji LAS 4000 Captures images; chemiluminescence; ethidium bromide; white light images
Ion Torrent PGM Next-generation sequencing
Ion Torrent Proton Next-generation sequencing
Nanodrop spectrophotometer RNA, DNA, and protein quantification

PCR clean hoods

HEPA-filtered hoods for preparing DNA plates

QIAcube Automation of Qiagen kits
RNA clean area Provides an RNase-free work area and tools for RNA extraction

Fees

Per Use Fees

  • Affymetrix GeneChip Microarrays: All users wishing to perform GeneChip experiments will pay for their own reagents and supplies, including GeneChips, which can be purchased through CGR. Additionally, CGR charges $100 per chip towards the expense of maintenance. WSU users pay only $50 per chip.
  • Agilent 2100 Bioanalyzer: Bioanalyzer chips are paid for by users as needed and can be run for $10/chip by WSU users. Most chips can be purchased through the CGR at cost for $30.
  • Applied Biosystems 7900HT Sequence Detection System: There is a $12 per plate charge for use of the ABI real-time thermocycler. Users pay for their own TaqMan or SYBR Green RT-qPCR kits, as well as tips, plates, and other supplies.
  • Accuri C6 Cytometer: Charges for the flow cytometer are still $30 per hour to cover sheath fluid and maintenance. Users should provide their own tubes and reagents.
  • Imaging Systems: The Fuji LAS-3000 and Amersham 600 RGB imagers are $1/gel or membrane.
  • Nanodrop Spectrophotometer: The two Nanodrop instruments are $1/sample.
  • Next Generation Sequencing: Please contact CGR personnel to budget NGS experiments.

It is the goal of the Center for Genomics Research to remain accessible to all Wright State customers. Users who feel unable to afford these fees should contact Dr. Michael Markey at michael.markey@wright.edu.